Genotyping by Sequencing for Crop Improvement. Группа авторов

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the designation of GG for Oryza granulata and HHJJ for Oryza ridleyi (Aggarwal et al. 1997 ).

      1.7.3 Deployment of Molecular Markers in Plant Variety Protection and Registration

      The current system of plant variety protection and registration using assessment of DUS (Distinctness, Uniformity, and Stability) characteristics predominantly relies upon morphological traits which are quite laborious, time‐consuming, requires skills, expertise, and evaluation under special designs for most quantitative traits. With the advent of novel breeding technologies, new varieties differ only for few traits or at few loci which make the process of detecting distinctness in varieties, a challenging task. Even with increasing numbers of plant varieties, DUS testing is becoming quite expensive. In a review by Jamali et al. (2019), DNA‐based molecular markers have been proposed to be a reliable alternative for conducting DUS testing. Molecular markers not only cut cost, time and labor but will also help in the proper sharing of Plant Breeder’s Rights with an assessment of few distinct traits, particularly in essentially derived varieties.

      The DNA‐based molecular markers are widely recognized for their enormous potential in plant breeding and genetic studies. The past few years have seen remarkable developments in the field of molecular markers technology particularly with the emergence of NGS technologies. SNPs have become the choice of markers of present and future based on their genome‐wide abundance, high polymorphism, amenability to high‐throughput automation, and easier analysis. SNPs can be utilized in different genotyping platforms such as GBS, DArT, WGR, SNP arrays, and KASP. Any genotyping platform can be chosen based on the objective of the study and cost concerns. As an example, GBS, WGR, SNP arrays, and KASP can generate similar type of results for genetic diversity analysis; however, KASP and GBS could be cheaper than others. While the development of KASP will depend upon the availability of SNPs particularly in SNP databases, but GBS can be done without any previous information. It has been observed that these recent marker genotyping technologies have accelerated the crop improvement programs particularly in the identification and utilization of novel genes and QTLs.

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