WASHINGTON, DC

      The two of us thank our life partners, Marcia Glick and Patrick Patten, for the enormous support and encouragement that they have provided throughout this endeavor

      B. R. Glick

      C. L. Patten

      Copyright © 2017 American Society for Microbiology. All rights reserved. No part of this publication may be reproduced or transmitted in whole or in part or reused in any form or by any means, electronic or mechanical, including photocopying and recording, or by any information storage and retrieval system, without permission in writing from the publisher.

      Disclaimer: To the best of the publisher’s knowledge, this publication provides information concerning the subject matter covered that is accurate as of the date of publication. The publisher is not providing legal, medical, or other professional services. Any reference herein to any specific commercial products, procedures, or services by trade name, trademark, manufacturer, or otherwise does not constitute or imply endorsement, recommendation, or favored status by the American Society for Microbiology (ASM). The views and opinions of the author(s) expressed in this publication do not necessarily state or reflect those of ASM, and they shall not be used to advertise or endorse any product.

      Library of Congress Cataloging-in-Publication Data

      Names: Glick, Bernard R., author. | Patten, Cheryl L., author.

      Title: Molecular biotechnology : principles and applications of recombinant DNA / Bernard R. Glick, Cheryl L. Patten.

      Description: 5th edition. | Washington, DC : ASM Press, [2017]

      Identifiers: LCCN 2017011321 | ISBN 9781555819361 (hardcover) | ISBN 9781683673101 (ebook)

      Subjects: LCSH: Biotechnology. | Genetic engineering. | Molecular biology.

      Classification: LCC TP248.2 .G58 2017 | DDC 660.6—dc23

      LC record available at https://lccn.loc.gov/2017011321

      All Rights Reserved

      Printed in Canada

      10 9 8 7 6 5 4 3 2 1

      Address editorial correspondence to

      ASM Press, 1752 N Street NW

      Washington, DC 20036-2904, USA

      Send orders to ASM Press, P.O. Box 605, Herndon, VA 20172, USA

      Phone: 800-546-2416; 703-661-1593

      Fax: 703-661-1501

      E-mail: [email protected]

      Online: http://www.asmscience.org

      doi: 10.1128/9781555819378

      Cover illustration: Terese Winslow, CMI

      Cover and interior design: Susan Brown Schmidler

      Illustrations: Patrick Lane, ScEYEnce Studios

      Cover Photo Credits

      Plant - © KYTan/Shutterstock.com

      Cells - © Jurlk Peter/Shutterstock.com

      Pills - © RidvanArda/Shutterstock.com

      Copper Bubbles - © tucsonazrealestateblog.com

      Contents

       Preface to the Fifth Edition

       1 The Development of Molecular Biotechnology

       Emergence of Molecular Biotechnology

       Recombinant DNA Technology

       Commercialization of Molecular Biotechnology

       Concerns and Consequences

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       2 Fundamental Technologies

       Molecular Cloning

       Preparation of DNA for Cloning

       Insertion of Target DNA into a Plasmid Vector

       Transformation and Selection of Cloned DNA in a Bacterial Host

       Cloning Eukaryotic Genes

       Recombinational Cloning

       Genomic Libraries

       Genome Engineering using CRISPR Technology

       Polymerase Chain Reaction

       Amplification of DNA by PCR

       Cloning PCR Products

       Quantitative PCR

       Chemical Synthesis of DNA

       Synthesis of Oligonucleotides