Molecular Biotechnology. Bernard R. Glick
Читать онлайн книгу.
Assembling Oligonucleotides into Genes
Sequencing using Reversible Chain Terminators
Sequencing by Single Molecule Synthesis
Preparation of Genomic DNA Sequencing Libraries
High-Throughput Next-Generation Sequencing Strategies
3 Production of Recombinant Proteins
Protein Production in Prokaryotic Hosts
Increasing Translation Efficiency
Facilitating Protein Purification
DNA Integration into the Host Chromosome
Heterologous Protein Production in Eukaryotic Cells
Posttranslational Modification of Eukaryotic Proteins
General Features of Eukaryotic Expression Systems
Baculovirus–Insect Cell Expression Systems
Mammalian Cell Expression Systems
Examples of Protein Engineering
Immunological Approaches to Detect Protein Biomarkers
Agglutination
Enzyme-Linked Immunosorbent Assays
Protein Arrays to Detect Polygenic Diseases
Immunoassays for Protein Conformation-Specific Disorders
DNA-Based Diagnostic Approaches
Hybridization Probes
PCR-Based Detection Methods
DNA Microarrays
Whole Genome Sequencing to Assess Genetic Disease Risk
Detecting RNA Signatures of Disease
Detection of Disease-Associated Changes in Gene Expression Using Microarrays
Detection of RNA Signatures of Antibiotic Resistance in Bacteria
Detection of miRNA Signatures of Cancers
Biofluorescent and Bioluminescent Systems
Fluorescent Proteins
Luciferase
Microbial Biosensors
SUMMARY
REFERENCES
REVIEW QUESTIONS
Pharmaceuticals
Human Interferons
Human Growth Hormone
Tumor Necrosis Factor Alpha
Extending Protein Half-Life
Enzymes