Recent Advances in Polyphenol Research. Группа авторов

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plant resources (Schmidt and Cheng 2017), but these trust issues frequently prevent outsiders from gaining access into traditional ecological knowledge about culturally important species. In order to bridge these concerns, our collaborating laboratory teams from three US universities (www.gibex.org) joined together to produce a portfolio of mobile biodiscovery kits. The interactive experiments in the kits enable participation of both elders and youth in bioexploration and therapeutic lead discovery, focusing on indigenous local plants with a history of medicinal use in traditional ecological knowledge (Kellogg et al. 2010; Flint et al. 2011; McOliver et al. 2015). This unconventional approach provided a sustainable and equitable entrée into traditional cultures and local healing practices, and allowed us to interface with tribal groups in Africa, Asia, and the Americas to create a bridge between traditional knowledge and chemical/physical science discovery experiments.

      Following the leads provided by naturopathic healers in various global settings, the mobile biodiscovery approach was used to shed light on the mechanistic properties of some of the traditionally wildcrafted polyphenol‐rich plants.

      3.5.1 Phlorotannins in Alaskan Seaweeds/Marine Algae

Module ID Approximate workshop time required to conduct the bioassays/procedures How long before results can be evaluated Relevant‐disease targets
Field collection* 2–3 hours n/a n/a
Plant material extraction 1–2 hours n/a n/a
Antibacterial assay 2–3 hours 24–48 hours Bacterial pathogens
Antioxidant assay 1 hour Immediate General health
Glucosidase and glucosidase inhibitor assays 2 hours Immediate Metabolic syndrome type 2 diabetes
α‐amylase inhibition 1–2 hours Immediate Metabolic syndrome type 2 diabetes
Anthocyanin detection 1 hour Immediate General health
Protease and protease inhibitor assay 30 minutes–1 hour 10 minutes Digestive diseases Viral pathogens
Planaria lethality assay 1–2 hours 8–24 hours Parasitic worms
Planaria regeneration assay 1–2 hours 5 days Parasitic worms
Nematode lethality assay 1 hour 4 hours Parasitic worms

      * Field identification of medicinal plants and sample collection are conducted in concert with local elders from the community.

Photo depicts an Alaska Native youth engaging in a workshop featuring simple mobile biodiscovery assays during a 2019 fish camp on the Yukon River in Alaska.