1  Cover

      2  Title Page

      3  Copyright Page

      4  Foreword

      5  About the companion website

      6  Introduction

      7  Chapter 1: General Aspects of Chromatography INTRODUCTION 1.1 GENERAL CONCEPTS OF ANALYTICAL CHROMATOGRAPHY 1.2 THE CHROMATOGRAM 1.3 GAUSSIAN PEAKS AND REAL PEAKS 1.4 PLATE THEORY 1.5 NERNST PARTITION COEFFICIENT (K) 1.6 COLUMN EFFICIENCY 1.7 RETENTION PARAMETERS 1.8 SEPARATION (OR SELECTIVITY) FACTOR 1.9 RESOLUTION FACTOR 1.10 INFLUENCE OF SPEED OF THE MOBILE PHASE 1.11 OPTIMIZATION OF A CHROMATOGRAPHIC ANALYSIS 1.12 CLASSIFICATION OF CHROMATOGRAPHIC TECHNIQUES 1.13 PRINCIPLE AND BASIC RELATIONSHIP 1.14 CHROMATOGRAPHY SOFTWARE 1.15 EXTERNAL STANDARD METHOD 1.16 INTERNAL STANDARD METHOD 1.17 INTERNAL NORMALIZATION METHOD KEY POINTS OF THE CHAPTER PROBLEMS SOLUTIONS

      8  Chapter 2: Gas Chromatography 2.1 COMPONENTS OF A GC INSTALLATION 2.2 CARRIER GAS AND FLOW REGULATION 2.3 INJECTION CHAMBER 2.4 TEMPERATURE‐CONTROLLED OVEN 2.5 COLUMNS 2.6 STATIONARY PHASES 2.7 MAIN DETECTORS 2.8 OPTIMIZATION OF A SEPARATION 2.9 FAST CHROMATOGRAPHY AND MICROCHROMATOGRAPHY 2.10 RETENTION INDEXES AND STATIONARY PHASE CONSTANTS KEY POINTS OF THE CHAPTER PROBLEMS SOLUTIONS

      9  Chapter 3: High‐Performance Liquid Chromatography 3.1 DESIGN OF AN HPLC APPARATUS 3.2 PUMPS AND ELUTION GRADIENTS 3.3 INJECTORS 3.4 COLUMNS 3.5 STATIONARY PHASES 3.6 MOBILE PHASES 3.7 SPECIFIC COLUMNS 3.8 PRINCIPAL DETECTORS 3.9 HPLC OPTIMIZATION 3.10 NEW DEVELOPMENTS IN HPLC KEY POINTS OF THE CHAPTER PROBLEMS SOLUTIONS

      10  Chapter 4: Ion Chromatography 4.1 BASICS OF ION CHROMATOGRAPHY 4.2 STATIONARY PHASES FOR IEC 4.3 MOBILE PHASES 4.4 CONDUCTIVITY DETECTORS 4.5 WATER PEAK AND SYSTEM PEAK 4.6 ION SUPPRESSORS OF THE ELECTROLYTE 4.7 ION‐EXCLUSION CHROMATOGRAPHY 4.8 AMINO ACID ANALYSERS 4.9 PASSAGE FROM IEC TO ULTRA‐IEC KEY POINTS OF THE CHAPTER PROBLEMS SOLUTIONS

      11  Chapter 5: Thin‐Layer Chromatography 5.1 PRINCIPLE OF THIN‐LAYER CHROMATOGRAPHY (TLC) 5.2 CHARACTERISTICS OF TLC 5.3 STATIONARY PHASES 5.4 SEPARATION AND RETENTION