anti‐Sarcoptes antibodies in serum. Positive results indicate possible Sarcoptes infection, or false positive due to house dust mite hypersensitivity (these antigens cross react). Dogs may remain positive for up to 6 months after successful treatment. Negative results indicate lack of exposure, early infection, or immunosuppressive medication interactions. Sensitivity is 84.2%, and specificity is 89.5%
Cornell University College of Veterinary Medicine, Animal Health Diagnostic Center PCR
Indicates the presence of DNA from Ehrlichia canis, Neorickkettsia risticii, Anaplasma phagocytophilum, Bartonella spp., hemotropic Mycoplasma spp. Many additional individual PCR tests available on website
Indicates the presence of DNA from Anaplasma spp., Babesia spp., Bartonella spp., canine hemotropic Mycoplasma, Ehrlichia spp., Hepatozoon spp., Leishmania spp., Neorickettsia risticii, and Rickettsia rickettsii. Quantifies DNA of Leishmania spp.
Measures antibodies to Borrelia burgdorferi, Anaplasma sp., and Ehrilichia sp., and measures Dirofilaria immitis antigen Measures presence of DNA from several tick‐borne organisms, and measures Borrelia burgdorferi antibodies. Also offers B. burgdorferi PCR and Western Blot
Provided by many research institutions and universities. Polymerase chain reaction (PCR) tests are available for common dermatologically relevant bacteria, including Staphylococcus spp., Pseudomonas sp., Streptococcus sp., E. coli, Bordetella sp., Mycobacteria spp., Bartonella sp., etc. Refer to diagnostic laboratories at large research institutions nearest you for more information, especially in regard to each test's sensitivity and specificity. While most PCRs are highly sensitive and specific, remember that the presence of DNA can represent contamination of the sample or the presence of nonviable bacteria. Results should be correlated with clinical findings, cytology, histopathology, and even culture when possible
Measures antibodies to various fungi, including Coccidioides sp., Histoplasma sp., and others. Measures presence of Cryptococcus sp. antigen (latex agglutination) and Blastomyces sp. antigen (urine)
Provided by many research institutions and universities. PCRs are available for common dermatologically relevant fungi. Refer to diagnostic laboratories at large research institutions nearest you for more information, especially in regard to each test's sensitivity and specificity
Fungal PCR
IDEXX: Blastomyces dermatitidis RealPCR Test Systemic Mycoses RealPCR Test
Whole blood (EDTA) or deep pharyngeal swab Aspirated material or fresh tissue
Positive indicates the presence of Blastomyces dermatitidis DNA in sample and must be considered with presence of clinical signs consistent with infection. False negative is possible if sample is inadequate or due to antifungal therapy administration Positive indicates presence of DNA from Blastomyces dermatitidis, Coccidioides spp.,
