To Catch a Virus. John Booss

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bacterial infection was not present (57).

      Embryonated Eggs

      The use of experimental animals and that of embryonated eggs were the principal means of virus isolation prior to the development of cell culture in the 1950s. Animals were crucial to the isolation and characterization of herpes viruses, among many others. In parallel, development of serological techniques to measure virus-specific antibodies was to become the major means of viral diagnosis for several decades. That story is told in the next chapter.

      References

      1 Andrewes, C. H., P. P. Laidlaw, and W. Smith. 1934. The susceptibility of mice to the viruses of human and swine influenza. Lancet 224:859–882.