Bovine Reproduction. Группа авторов
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The normal bovine male reproductive system consists of paired testes retained within a sac or purse‐like structure known as the scrotum, which is formed from the outpouching of skin from the abdomen and consists of complex layers of tissue. The testes are accompanied by a number of supporting structures including spermatic cords, accessory sex glands (prostate, bulbourethral, paired vesicular glands), penis, prepuce, and the male ductal system. The testicular duct system is extensive and comprises the vas efferentia found within the testes, epididymis, vas deferens, and urethra, all of which are located external to the testes. The reader is referred to the chapter on the anatomy of the reproductive system of the bull in this book (Chapter 1). The primary functions of the testes are to produce male gametes (spermatozoa) and the endocrine factors, such as steroid (testosterone) and protein hormones (inhibin, insulin‐like peptide 3), which help regulate the reproductive function of the bull in concert with hormonal secretions from the hypothalamus (gonadotropin‐releasing hormone) and pituitary glands (luteinizing hormone (LH), follicle‐stimulating hormone (FSH)). The testes consist of parenchymal tissue that supports the interstitial tissue and includes the steroid‐producing Leydig cells, vascular and lymphatic system, and seminiferous tubules within which the germinal tissue develops with the support of the nurse cells more commonly known as Sertoli cells. Chapter 5 discusses in detail the endocrine factors responsible for testicular development and initiation of spermatogenesis in the bull, and thus this chapter focuses more on the regulation and function of the adult testes. This chapter will not undertake a treatise of those conditions that disrupt testicular function but rather will focus, as practically as is possible, on what is known of the endocrine and exocrine function of the bovine testes. Much of the endocrine and exocrine function of the testes is similar across mammalian species, and where specific information is absent for the bovine, examples will be given from other domestic species when possible. It has not been possible to cite the many significant contributions to the field of endocrine and exocrine function of the testes. Thus, where and when possible, the reader is referred to selected citations for additional reading.
Historical Perspective
It has been evident for many centuries that the testes exercise control over the characteristics of the male body. The results of castration in domestic animals and human males made this very clear, but provided no clues as to the mechanism of control. Pritchard [1] noted from Assyrian records dating some 15 centuries BCE that the castration of men was used as punishment for sexual offenders, which suggests that the effect of castration on fertility and behavior was recognized at that time. Knowledge of the effects of castration of livestock dates back to the Neolithic Age (c. 7000 BCE) when animals were first thought to have been domesticated [2]. The effects of castration were understood by Aristotle (~300 BCE) who provided very detailed and clear descriptions of testicular anatomy and function [3]. It was not until the seventeenth century that a detailed account of testicular and penile anatomy was presented by Regnier de Graaf [4] in a treatise on the male reproductive organs. De Graaf indicated the existence of the seminiferous tubules and suggested that the production of the fertile portion of the semen occurred in the testes. The first microscopic examination of the testes was undertaken by Antonie van Leeuwenhoek in 1667 where he demonstrated and reported the presence of germ cells in the seminal fluid [5].
Detailed study of the testis began in the mid‐nineteenth century. In 1840, Albert von Köllicker discovered that spermatozoa develop from cells residing in the testicular (seminiferous) tubules. This major discovery was followed by Franz Leydig's [6] description of the microscopic characteristics of the interstitial cells. Later, Enrico Sertoli [7], an Italian scientist, correctly described the columnar cells running from the basement membrane to the lumen of the tubuli seminiferi contorti (seminiferous tubules) of the testes, and Anton von Ebner is credited with introducing the concept of the symbiotic relationship between Sertoli cells and the developing germinal cells [8, 9].
The first clear demonstration that the testes are involved in an endocrine role was made by Arnold Berthold in 1849, while studying the testes of the rooster. He concluded that the regulation of male characteristics was brought about by way of blood‐borne factors. The most compelling evidence for an endocrine function of the testes being associated with Leydig cells was presented by two French scientists, Bouin and Ancel in 1903. They reported that ligation of the vas deferens in dogs, rabbits, and guinea pigs was followed by degeneration of the seminiferous tubules, but no castration effects were observed and no degenerative changes of the interstitial cells, and thus it was concluded that internal secretions of the testes were synthesized by the Leydig cells [10]. By the mid‐1930s it was clear that the male hormone emanating from the testes was testosterone [11] and that the function of the testes was controlled by pituitary hormones [12, 13]. Smith [12] demonstrated that the pituitary gland must secrete substances (now known as gonadotropins) responsible for the stimulation of testicular growth and maintenance of function in the rat. Greep et al. [14] restored Leydig cell function in hypophysectomized rats with crude preparations of LH and reestablished the male secondary sex characteristics. Further evidence was elucidated in favor of a steroid secretory function for Leydig cells from a study on postnatal development in bulls where changes in testicular androgen levels paralleled the differentiation of these cells [15]. Using cell culture techniques, Steinberger et al. [16] provided direct evidence that the Leydig cells are the primary source of steroid hormone synthesis in the testes. Later, it became apparent that the Leydig cells are essential for providing the androgenic stimuli that are required for the maintenance of spermatogenesis in the germinal epithelium [16, 17].
The Testis
The bovine testes are paired, capsulated, ovoid‐like structures located in the inguinal region and suspended in a pendulous scrotum away from the abdominal wall. The proximal relationship of the testes to the abdominal wall varies and may depend on season and ambient temperatures. The cremaster muscle plays an important role in thermal regulation of the testis. The size of the testis varies with breed, but typically the adult testis weighs 300–400 g and is about 10–13 cm long and 5–6.5 cm wide [18]. The tough fibrous capsule covering each testis consists of three tissue layers: the outer layer, the tunica vaginalis; the tunica albuginea, which consists of connective tissue composed of fibroblasts and collagen bundles; and the inner layer, the tunica vaginalis, which supports the vascular and lymphatic systems [19]. The capsule is the main structure that supports the testicular parenchyma, the functional layer of the testes, which consists of the interstitial tissue and seminiferous tubules. The interstitial tissue is found in the spaces between the seminiferous tubules and consists of clusters of Leydig cells, which are primarily responsible for steroid hormone biosynthesis and secretion, along with vascular and lymph vessels that supply the testicular parenchyma. The seminiferous tubules originate from the primary sex cords and contain the germinal tissue (spermatogonia, the male germ cell) and a population of specialized cells, the Sertoli cells, which not only support the production of spermatozoa but also form tight junctions with each other, creating one of the most important components of the blood–testis barrier [20]. This structure prevents the entry of most large molecules and foreign material into the seminiferous tubules that may disrupt normal spermatogenesis. The most important substances synthesized by the testes and released into the vascular system are peptide and steroid hormones. However, fluids from the seminiferous tubules may pass into the interstitial tissue via the basal lamina, where they may enter the testicular lymphatic and vascular systems, or into the tubule lumen via the apical surface of the Sertoli cells [19].
The Scrotum and Spermatic Cords
The scrotum is composed of an outer layer of thick skin and three underlying layers, the tunica dartos, the scrotal fascia, and the parietal vaginal tunica. The scrotal skin is extensively populated with numerous large adrenergic sweat and sebaceous glands that are highly endowed with thermal receptors and nerve fibers. Neural stimulation from the thermal receptors enables the tunica dartos, which consists of smooth muscle fibers and lies just beneath the scrotal skin, to contract and relax in response to changes in temperature gradients and facilitates